What the Cell
A2.2

Cell structure

3 hours11 statementsTextbook pp. 49–81

Guiding questions

  1. What do all cells share, and how do they differ?
  2. How do we use microscopes to study cell structure?

Slides

Slide 1 of 22

Notes

Written for this site from the class slides. The chapter is on pages 49–81 of the course textbook. Image credits are on slide 22.

Cells and microscopes

A2.2.1

Cells: the basic unit of life

pp. 49–81

All organisms are built from cells, the basic unit of structure. Using deductive reasoning, cell theory predicts that a newly discovered organism will consist of one or more cells.

A2.2.2

Microscopy skills

pp. 49–81

Practise making temporary mounts, staining, focusing with coarse and fine adjustment, and measuring with an eyepiece graticule. Magnification = image size / actual size. For example, a cell 8 µm wide drawn 40 mm wide has a magnification of 40 000 / 8 = ×5000.

Watch out: convert to the same units first. 1 mm = 1000 µm.

A2.2.3

Developments in microscopy

pp. 49–81
TechniqueWhy it is useful
Electron microscopyElectrons have a shorter wavelength, so resolution is far higher
Fluorescent stainsShow chosen structures in colour
ImmunofluorescenceAntibodies carry a dye to one specific target
Freeze fractureFrozen cells split along the middle of membranes, revealing surfaces
Cryogenic electron microscopyRapid freezing preserves molecules, so that 3D models can be made

Types of cell

A2.2.4

Structures in all cells

pp. 49–81

All cells have DNA as genetic material, cytoplasm composed mainly of water, where reactions take place, and a plasma membrane of lipids, which separates inside from outside.

A2.2.5

Prokaryotic cells

pp. 49–81

A Gram-positive eubacterium, such as Bacillus or Staphylococcus, has a cell wall, plasma membrane, cytoplasm, naked DNA in a loop, and 70S ribosomes.

Slide: diagram of a prokaryote, with a table of its structures
A2.2.6

Eukaryotic cells

pp. 49–81

A plasma membrane encloses a compartmentalized cytoplasm, with 80S ribosomes. The nucleus has a double membrane with pores, and chromosomes of DNA bound to histones. There is a cytoskeleton of microtubules and microfilaments.

OrganelleFunction
MitochondrionAerobic respiration
Rough ERMakes proteins for secretion or membranes
Smooth ERMakes lipids
Golgi apparatusModifies and packages proteins
LysosomeDigests food and old organelles

Life and variety

A2.2.7

Life in a single cell

pp. 49–81

Single-celled organisms carry out homeostasis, metabolism, nutrition, movement, excretion, growth, response to stimuli and reproduction. In Paramecium, for example, contractile vacuoles expel water, food vacuoles digest food, and cilia provide movement.

A2.2.8

Animals, fungi and plants

pp. 49–81
AnimalsFungiPlants
Cell wallNoneChitinCellulose
VacuoleSmall, temporaryLarge or smallLarge, permanent
PlastidsNoneNoneChloroplasts and others
Centrioles, cilia, flagellaOften presentMostly noneMostly none
A2.2.9

Atypical cell structure

pp. 49–81

Many nuclei: aseptate fungal hyphae and skeletal muscle. No nucleus: mature red blood cells and phloem sieve tube elements.

Skills

A2.2.10

Reading micrographs

pp. 49–81

A prokaryote is small, with a nucleoid and a cell wall. A plant cell has a cell wall, chloroplasts and a large vacuole. An animal cell has a nucleus and mitochondria, but no cell wall. Practise finding each organelle in electron micrographs.

A2.2.11

Drawing and annotating

pp. 49–81

Use a sharp pencil and clear, continuous lines, without shading. Keep to the proportions of the micrograph. Annotate each structure with a function: for example, microvilli increase the surface area for absorption.

Videos

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Note-taking sheet

Print this guide and complete it as you work through the slides and videos. It asks for your own explanations, so close the notes while you try each section.

First page of the note-taking sheet

Quick self-check

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Practice questions

A printable worksheet covering the chapter, from quick recall to calculations, drawings and a longer answer. It has 48 marks and takes about 50 minutes, or you can do one section at a time.

Your teacher has the mark scheme.

First page of the worksheet

Practical

Drawing and measuring onion cells

Lab45 minA2.2.2

You will need: Onion, iodine solution, slides, coverslips, forceps, microscope, stage micrometer or ruler

  1. Peel a thin layer of onion epidermis and mount it in a drop of iodine
  2. Lower the coverslip gently to avoid air bubbles
  3. Draw several cells with a sharp pencil and label visible structures
  4. Estimate cell length and calculate the magnification of your drawing

Safety: Iodine stains and irritates, so wash off any skin contact. Take care with glass.

Carry this out with your teacher's supervision and follow your school's lab rules.

Nature of science and TOK

Syllabus statements

Summarised in our own words. Use each code to find the exact wording in your textbook or the IB subject guide.

  • A2.2.1The cell as the basic building block of all organismsp. 63
  • A2.2.2Practical skills for using a microscopep. 52
  • A2.2.3Advances in microscopyp. 60
  • A2.2.4The structures found in the cells of every organismp. 63
  • A2.2.5The structure of a prokaryotic cellp. 65
  • A2.2.6The structure of a eukaryotic cellp. 67
  • A2.2.7How a single-celled organism carries out all the functions of lifep. 73
  • A2.2.8How animal, fungal and plant cells differ in structurep. 75
  • A2.2.9Eukaryotic cells with unusual structuresp. 78
  • A2.2.10Recognising cell types and structures in light and electron micrographsp. 79
  • A2.2.11Drawing and annotating cells from electron micrographsp. 80

HL extension

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