What the Cell
B3.1

Gas exchange

Plant
2 hours4 statementsTextbook pp. 283–291

Guiding questions

  1. What adaptations let multicellular organisms exchange gases?
  2. How do flowering plants and mammals differ, and resemble each other, in gas exchange?

Slides

Slide 1 of 16

Notes

Written for this site from the course textbook and class slides. Use the page numbers to read the book's fuller account. Gas exchange in animals (B3.1.1–6) is in Body Systems. Image credits are on slide 16.

The leaf

B3.1.7

How leaves are adapted to exchange gases

pp. 283–286

A leaf must take in carbon dioxide for photosynthesis, while limiting its loss of water. Being flat and thin gives it a large surface area and short diffusion distances.

StructureAdaptation
Waxy cuticleA largely impermeable layer, so gases and water vapour must pass through stomata, where flow is controlled
EpidermisMost pores are in the lower epidermis, which is shaded and cooler, and this reduces water loss
Air spacesLet gases circulate between loosely packed cells, maintaining gradients
Spongy mesophyllA large, moist surface for gases to enter and leave cells
Stomatal guard cellsOpen and close the pore, controlling gas exchange and water loss
VeinsXylem supplies water, and phloem removes sugars, which helps to maintain gradients

When guard cells take up water they become turgid and curve apart, opening the pore. When they lose water they become flaccid and the pore closes.

Slide: micrograph of a stoma and guard cells, with key points

Watch out: the stoma is the pore, and the guard cells are the cells around it.

B3.1.8

How tissues are arranged in a leaf

pp. 284–286

From top to bottom, a dicot leaf has a waxy cuticle, upper epidermis, palisade mesophyll, spongy mesophyll and lower epidermis with stomata. Veins, containing xylem and phloem, run through the mesophyll.

A plan diagram shows these tissues as regions. Use clear, continuous lines, do not shade, and do not draw individual cells.

Slide: schematic plan diagram showing the layers of a leaf

Losing water

B3.1.9

Why gas exchange in a leaf leads to transpiration

pp. 286–288

For carbon dioxide to enter, stomata must be open. Water evaporates from moist cell walls into the air spaces, and water vapour diffuses out through the same pores. This loss is transpiration.

FactorEffect of an increaseWhy
TemperatureRate risesMore evaporation, so a greater concentration difference of water vapour
HumidityRate fallsThe air outside holds more water vapour, so the gradient is smaller
WindRate risesWater vapour near the stomata is swept away, keeping the gradient steep
LightRate risesStomata open for photosynthesis, and the leaf warms

A potometer measures the rate at which a shoot takes up water, using the movement of an air bubble along a capillary tube. A small amount of this water stays in the plant, so it is an indirect measure.

Watch out: water vapour diffuses out, not liquid water, and this step is not osmosis.

B3.1.10

How many stomata a leaf has

pp. 289–291

Stomatal density is the number of stomata per unit area of leaf. More stomata can take up more carbon dioxide, but they can also lose more water.

  1. Paint clear nail varnish on the underside of a leaf, and let it dry.
  2. Lift it off with clear tape, and stick it to a slide.
  3. Count the stomata in several fields of view, and find the mean.
  4. Area of field = 3.14 × r². Density = mean count ÷ area.

Videos

Select a video to play it here. Select it again to close it.

Note-taking sheet

Print this guide and complete it as you work through the slides and videos. It asks for your own explanations, so close the notes while you try each section.

First page of the note-taking sheet

Quick self-check

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Practice questions

A printable worksheet covering all four statements, from quick recall to data questions. It has 35 marks and takes about 35 minutes, or you can do one section at a time.

Your teacher has the mark scheme.

First page of the worksheet

Data-based questions

Questions built on realistic experimental data, in the style of Paper 1B and Paper 2. Work them on paper and show your working. 6 marks in total. Your teacher has the mark scheme.

Carbon dioxide uptake by a succulent

6 marks

Aeonium is a succulent shrub from the Canary Islands. Net carbon dioxide uptake by one plant was measured over 24 hours, when well watered and after two weeks without water. It was dark from 18:00 to 06:00. Negative values mean carbon dioxide was released.

Line graph over 24 hours. Watered plant takes up carbon dioxide all day and night, with peaks around 04:00 and 12:00. Drought plant takes up carbon dioxide only at night, peaking at 1.6 at 04:00, and releases a little by day.
  1. State the time of the highest carbon dioxide uptake by the drought plant. [1]
  2. Compare the watered and drought plants during the day (06:00 to 18:00). [2]
  3. Explain why the uptake of the drought plant is negative at midday. [1]
  4. Suggest two advantages of taking up carbon dioxide at night in dry conditions. [2]

Practical

Stomatal density from leaf impressions

Lab40 minB3.1.10

You will need: Fresh leaves, clear nail varnish, clear tape, slides, microscope

  1. Paint a small patch on the underside of a leaf and let it dry
  2. Lift the dried film with clear tape and stick it to a slide
  3. Count stomata in the field of view at high power, in five fields
  4. Calculate stomata per square millimetre, then compare with the upper surface

Safety: Use nail varnish in a ventilated room, away from flames.

Carry this out with your teacher's supervision and follow your school's lab rules.

Nature of science and TOK

Syllabus statements

Summarised in our own words. Use each code to find the exact wording in your textbook or the IB subject guide.

  • B3.1.7How leaves are adapted to exchange gasesp. 283
  • B3.1.8How tissues are arranged in a leafp. 286
  • B3.1.9Why gas exchange in a leaf leads to transpirationp. 286
  • B3.1.10How many stomata a leaf has (stomatal density)p. 289

HL extension

More of B3.1 in this course

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